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KMID : 1000620030010020013
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International Journal of Vascular Biomedical Engineering
2003 Volume.1 No. 2 p.13 ~ p.19
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Analysis of Blood Flow-dependent Blood Nitric Oxide Level and Half-life of Nitric Oxide in Vivo
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Kim Cuk-Seong
Jeon Byeong-Hwa
Kim Hyo-Shin
Lee Young-Jun
Park Jin-Bo-Ry
Ryoo Sung-Woo
Chang Seok-Jang
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Abstract
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Endothelial release of nitric oxide (NO) contributes to the regulation of vascular tone by inducing vascular relaxation. To estimate the blood flow-dependent nitric oxide level and half-life (T1/2) of nitric oxide in vivo state, we investigated the change of aortic NO currents during the change of aortic blood flow rate using NO-selective electrode system and electromagnetic flowmeter in the aorta of anesthetized rats. Resting mean aortic blood flow rate was in the anesthetized rats. NO currents in the aorta were increased by the elevation of blood pressure and/or blood flow rate. When the aortic blood flow was occluded by the clamping, aortic NO currents were decreased. The difference of NO concentration between resting state and occluded state was (n=7). This NO concentration was estimated as blood flow-dependent nitric oxide concentration in the rats. Also, while the aortic blood flow was occluded, NO currents were decreased with exponential pattern with seconds of time constant and seconds of half-life. To summarize, this study suggested that blood flow-dependent NO concentration and half-life of nitric oxide were about and 7.7 seconds, respectively, in the aorta of anesthetized rats. The nitric oxide-selective electrode system is useful for the direct and continuous measurement of NO in vivo state.
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KEYWORD
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Nitric Oxide, Blood Flow, NO-selective electrode, Half-life, NO decay rate
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